Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA)
Somatic embryogenesis represents an essential tool for the genetic improvement and for the mass clonal micropropagation of the improved banana plant. In this present work morphological and anatomical patterns were analyzed in the somatic embryogenesis of Williams banana, to know and enhance this pro...
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Universidad Nacional de Colombia: Instituto de Biotecnología
2012
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| author | Ramírez Villalobos, Maribel García, Eva de Lindorf, Helga |
| author_browse | García, Eva de Lindorf, Helga Ramírez Villalobos, Maribel |
| author_facet | Ramírez Villalobos, Maribel García, Eva de Lindorf, Helga |
| author_sort | Ramírez Villalobos, Maribel |
| building | Dialnet |
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| description | Somatic embryogenesis represents an essential tool for the genetic improvement and for the mass clonal micropropagation of the improved banana plant. In this present work morphological and anatomical patterns were analyzed in the somatic embryogenesis of Williams banana, to know and enhance this process. In the investigation embryogenic cell suspensions (ECS) were established from embryogenic callus obtained from floral immature male hands, which gave rise to many somatic embryos that regenerated plants. Towards the three months of culture white-cream primary somatic embryos (SE) were detected in the floral hands of the nodes nine to twelve, counted from the floral apex. At the fourth month this primary SE gave origin to a creamy-white embryogenic callus, with granular structure and abundant SE torpedo on its periphery. Cell ubiorganization
with three different zones was observed in callus. Small portions of this callus were cultivated in the multiplication medium for two months, to originate ECS I. This ECS was filtered through a mesh (250 ìm pore size) to establish the ECS II. The sediment of embryogenic cells and cell clusters of the ECS were moved to maturation media. After two months the mature embryos were transferred to conversion medium, and two weeks later, whole plants were developed. The ECS produced numerous mature SE, which showed good conversion of embryos into plants and plant regeneration. This system of somatic embryogenesis permitted the mass production of functional plants in nine months. |
| first_indexed | 2026-10-16T04:16:23Z |
| format | Article |
| fullrecord | <oai_dc:dc xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><identifier>dialnet-ART0000545430</identifier><datestamp>2022-02-17</datestamp><dc:title>Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA)</dc:title><dc:creator>Ramírez Villalobos, Maribel</dc:creator><dc:creator>García, Eva de</dc:creator><dc:creator>Lindorf, Helga</dc:creator><dc:subject>Musa</dc:subject><dc:subject>callo embriogénico</dc:subject><dc:subject>suspensiones celulares embriogénicas</dc:subject><dc:subject>embriones maduros</dc:subject><dc:subject>conversión de embriones</dc:subject><dc:subject>Musa</dc:subject><dc:subject>embryogenic callus</dc:subject><dc:subject>embryogenic cell suspension</dc:subject><dc:subject>mature embryos</dc:subject><dc:subject>conversion of embryos</dc:subject><dc:description>Somatic embryogenesis represents an essential tool for the genetic improvement and for the mass clonal micropropagation of the improved banana plant. In this present work morphological and anatomical patterns were analyzed in the somatic embryogenesis of Williams banana, to know and enhance this process. In the investigation embryogenic cell suspensions (ECS) were established from embryogenic callus obtained from floral immature male hands, which gave rise to many somatic embryos that regenerated plants. Towards the three months of culture white-cream primary somatic embryos (SE) were detected in the floral hands of the nodes nine to twelve, counted from the floral apex. At the fourth month this primary SE gave origin to a creamy-white embryogenic callus, with granular structure and abundant SE torpedo on its periphery. Cell ubiorganization
with three different zones was observed in callus. Small portions of this callus were cultivated in the multiplication medium for two months, to originate ECS I. This ECS was filtered through a mesh (250 ìm pore size) to establish the ECS II. The sediment of embryogenic cells and cell clusters of the ECS were moved to maturation media. After two months the mature embryos were transferred to conversion medium, and two weeks later, whole plants were developed. The ECS produced numerous mature SE, which showed good conversion of embryos into plants and plant regeneration. This system of somatic embryogenesis permitted the mass production of functional plants in nine months.</dc:description><dc:description>La embriogenesis somatica representa una herramienta esencial en el mejoramiento genetico y en la micropropagacion clonal masiva de bananos mejorados. En el presente trabajo se analizaron los patrones morfologicos y anatomicos que ocurren durante la embriogenesis somatica del banano Williams, dirigidos a conocer y mejorar este proceso. En la investigacion se establecieron suspensiones celulares embriogenicas (SCE) a partir de callo embriogenico obtenido de manos florales inmaduras masculinas, las cuales originaron abundantes embriones que regeneraron plantas. Hacia los tres meses de cultivo se detectaron embriones somaticos (ES) primarios color blanco-crema en las manos florales de los nudos nueve a doce, contados a partir del apice floral. Al cuarto mes estos ES primarios dieron origen al callo embriogenico, de color blanco crema, estructura granular, con abundantes ES torpedo en su periferia y con una organizacion celular en tres diferentes zonas. De este callo se cultivaron porciones pequenas con ES torpedo en medio de multiplicacion durante dos meses, dando origen a la SCE I. La misma se tamizo (250 Êm) para establecer la SCE II. El sedimento de celulas y los agregados celulares embriogenicos de ambas SCE se traslado a medio de maduracion. Transcurridos dos meses los embriones maduros se transfirieron a medio de conversion de embriones, lograndose regenerar plantas completas a partir de las dos semanas. Las SCE produjeron numerosos embriones somaticos maduros y mostraron una buena conversion de embriones a plantas y regeneracion de plantas. Este sistema de embriogenesis somatica permitio la obtencion de plantas funcionales en nueve meses.</dc:description><dc:publisher>Universidad Nacional de Colombia: Instituto de Biotecnología</dc:publisher><dc:date>2012</dc:date><dc:type>text (article)</dc:type><dc:format>application/pdf</dc:format><dc:identifier>https://dialnet.unirioja.es/servlet/oaiart?codigo=4193481</dc:identifier><dc:identifier>(Revista) ISSN 1909-8758</dc:identifier><dc:identifier>(Revista) ISSN 0123-3475</dc:identifier><dc:source>Revista Colombiana de Biotecnología, ISSN 0123-3475, Vol. 14, Nº. 1, 2012, pags. 41-52</dc:source><dc:language>spa</dc:language><dc:rights>LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI</dc:rights></oai_dc:dc> |
| id | dialnet-ART0000545430 |
| institution | Dialnet |
| language | spa |
| last_indexed | 2026-10-16T04:16:23Z |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Universidad Nacional de Colombia: Instituto de Biotecnología |
| publisherStr | Universidad Nacional de Colombia: Instituto de Biotecnología |
| record_format | oai_dc |
| spelling | dialnet-ART00005454302022-02-17Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA)Ramírez Villalobos, MaribelGarcía, Eva deLindorf, HelgaMusacallo embriogénicosuspensiones celulares embriogénicasembriones madurosconversión de embrionesMusaembryogenic callusembryogenic cell suspensionmature embryosconversion of embryosSomatic embryogenesis represents an essential tool for the genetic improvement and for the mass clonal micropropagation of the improved banana plant. In this present work morphological and anatomical patterns were analyzed in the somatic embryogenesis of Williams banana, to know and enhance this process. In the investigation embryogenic cell suspensions (ECS) were established from embryogenic callus obtained from floral immature male hands, which gave rise to many somatic embryos that regenerated plants. Towards the three months of culture white-cream primary somatic embryos (SE) were detected in the floral hands of the nodes nine to twelve, counted from the floral apex. At the fourth month this primary SE gave origin to a creamy-white embryogenic callus, with granular structure and abundant SE torpedo on its periphery. Cell ubiorganization with three different zones was observed in callus. Small portions of this callus were cultivated in the multiplication medium for two months, to originate ECS I. This ECS was filtered through a mesh (250 ìm pore size) to establish the ECS II. The sediment of embryogenic cells and cell clusters of the ECS were moved to maturation media. After two months the mature embryos were transferred to conversion medium, and two weeks later, whole plants were developed. The ECS produced numerous mature SE, which showed good conversion of embryos into plants and plant regeneration. This system of somatic embryogenesis permitted the mass production of functional plants in nine months.La embriogenesis somatica representa una herramienta esencial en el mejoramiento genetico y en la micropropagacion clonal masiva de bananos mejorados. En el presente trabajo se analizaron los patrones morfologicos y anatomicos que ocurren durante la embriogenesis somatica del banano Williams, dirigidos a conocer y mejorar este proceso. En la investigacion se establecieron suspensiones celulares embriogenicas (SCE) a partir de callo embriogenico obtenido de manos florales inmaduras masculinas, las cuales originaron abundantes embriones que regeneraron plantas. Hacia los tres meses de cultivo se detectaron embriones somaticos (ES) primarios color blanco-crema en las manos florales de los nudos nueve a doce, contados a partir del apice floral. Al cuarto mes estos ES primarios dieron origen al callo embriogenico, de color blanco crema, estructura granular, con abundantes ES torpedo en su periferia y con una organizacion celular en tres diferentes zonas. De este callo se cultivaron porciones pequenas con ES torpedo en medio de multiplicacion durante dos meses, dando origen a la SCE I. La misma se tamizo (250 Êm) para establecer la SCE II. El sedimento de celulas y los agregados celulares embriogenicos de ambas SCE se traslado a medio de maduracion. Transcurridos dos meses los embriones maduros se transfirieron a medio de conversion de embriones, lograndose regenerar plantas completas a partir de las dos semanas. Las SCE produjeron numerosos embriones somaticos maduros y mostraron una buena conversion de embriones a plantas y regeneracion de plantas. Este sistema de embriogenesis somatica permitio la obtencion de plantas funcionales en nueve meses.Universidad Nacional de Colombia: Instituto de Biotecnología2012text (article)application/pdfhttps://dialnet.unirioja.es/servlet/oaiart?codigo=4193481(Revista) ISSN 1909-8758(Revista) ISSN 0123-3475Revista Colombiana de Biotecnología, ISSN 0123-3475, Vol. 14, Nº. 1, 2012, pags. 41-52spaLICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI |
| spellingShingle | Musa callo embriogénico suspensiones celulares embriogénicas embriones maduros conversión de embriones Musa embryogenic callus embryogenic cell suspension mature embryos conversion of embryos Ramírez Villalobos, Maribel García, Eva de Lindorf, Helga Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA) |
| title | Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA) |
| title_full | Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA) |
| title_fullStr | Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA) |
| title_full_unstemmed | Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA) |
| title_short | Análisis de patrones morfológicos y anatómicos en la embriogénesis somática del banano Williams (AAA) |
| title_sort | analisis de patrones morfologicos y anatomicos en la embriogenesis somatica del banano williams aaa |
| topic | Musa callo embriogénico suspensiones celulares embriogénicas embriones maduros conversión de embriones Musa embryogenic callus embryogenic cell suspension mature embryos conversion of embryos |
| url | https://dialnet.unirioja.es/servlet/oaiart?codigo=4193481 |
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