Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary
Phytophthora infestans (Mont.) de Bary, has caused considerable losses in all areas where are cultivated the Solanaceae. Its control has been done by chemical, biological, and traditionally by farmers using sisal juice. We isolated bacteria present in the sisal juice. These were tested for antagonis...
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2014
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| author | Otero, Ivan Dario Hurtado Benavides, Andrés Mauricio Arango Bedoya, Oscar Ferandez, Pablo Martinez, Fernando Javier Parra, Zaira Stephanie |
| author_browse | Arango Bedoya, Oscar Ferandez, Pablo Hurtado Benavides, Andrés Mauricio Martinez, Fernando Javier Otero, Ivan Dario Parra, Zaira Stephanie |
| author_facet | Otero, Ivan Dario Hurtado Benavides, Andrés Mauricio Arango Bedoya, Oscar Ferandez, Pablo Martinez, Fernando Javier Parra, Zaira Stephanie |
| author_sort | Otero, Ivan Dario |
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| description | Phytophthora infestans (Mont.) de Bary, has caused considerable losses in all areas where are cultivated the Solanaceae. Its control has been done by chemical, biological, and traditionally by farmers using sisal juice. We isolated bacteria present in the sisal juice. These were tested for antagonism to P. infestans using the principle of Bauer-Kirby technique. The isolate with most inhibition zone was submeted to fermentation in sterile sisal juice and was also tested for antagonism against P. infestans on tomato agar amended with the fermented broth. Finally, we performed the molecular characterization of isolates selected using the 16S rRNA gene ribosomal. We obtained a total of 41 isolates, eigth of which showed good growth inhibition in vitro of P. infestans. The M46 Isolate was selected for testing of antagonism in tomato agar plates amended with 200μl of fermented broth and reached a rate of 95,4% inhibition of growth of P. infestans. The M46 isolate was identified as Bacillus sp M46 with a percentage identity of 85% with Bacillus cereus. The strain of Bacillus sp. M46 has potential as a control agent for the pathogen P. infestans. |
| first_indexed | 2026-10-16T04:41:10Z |
| format | Article |
| fullrecord | <oai_dc:dc xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/" xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><identifier>dialnet-ART0001218921</identifier><datestamp>2026-05-15</datestamp><dc:title>Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary</dc:title><dc:creator>Otero, Ivan Dario</dc:creator><dc:creator>Hurtado Benavides, Andrés Mauricio</dc:creator><dc:creator>Arango Bedoya, Oscar</dc:creator><dc:creator>Ferandez, Pablo</dc:creator><dc:creator>Martinez, Fernando Javier</dc:creator><dc:creator>Parra, Zaira Stephanie</dc:creator><dc:subject>Antagonism</dc:subject><dc:subject>Oomycete</dc:subject><dc:subject>Bacillus sp</dc:subject><dc:subject>Bauer-Kirby technique</dc:subject><dc:subject>Antagonismo</dc:subject><dc:subject>Oomycete</dc:subject><dc:subject>Bacillus sp</dc:subject><dc:subject>Técnica de Bauer-Kirby</dc:subject><dc:description>Phytophthora infestans (Mont.) de Bary, has caused considerable losses in all areas where are cultivated the Solanaceae. Its control has been done by chemical, biological, and traditionally by farmers using sisal juice. We isolated bacteria present in the sisal juice. These were tested for antagonism to P. infestans using the principle of Bauer-Kirby technique. The isolate with most inhibition zone was submeted to fermentation in sterile sisal juice and was also tested for antagonism against P. infestans on tomato agar amended with the fermented broth. Finally, we performed the molecular characterization of isolates selected using the 16S rRNA gene ribosomal. We obtained a total of 41 isolates, eigth of which showed good growth inhibition in vitro of P. infestans. The M46 Isolate was selected for testing of antagonism in tomato agar plates amended with 200μl of fermented broth and reached a rate of 95,4% inhibition of growth of P. infestans. The M46 isolate was identified as Bacillus sp M46 with a percentage identity of 85% with Bacillus cereus. The strain of Bacillus sp. M46 has potential as a control agent for the pathogen P. infestans.</dc:description><dc:description>Phytophthora infestans (Mont.) de Bary, causa pérdidas considerables en todas las áreas donde se cultivan Solanáceas. Su manejo se realiza por métodos químicos, biológicos y tradicionalmente por los agricultores utilizando jugo de fique. Se aislaron bacterias presentes en el jugo de fique y se evaluó el antagonismo contra P. infestans empleando el principio de la técnica de Bauer–Kirbyel. Se seleccionó el aislado con mayor inhibición para realizar una fermentación en jugo de fique estéril y pruebas de antagonismo contra P. infestans en cajas de agar tomate enmendadas con caldo fermentado. Finalmente, se realizó la caracterización molecular del aislado seleccionado mediante la secuencia parcial del gen ribsomal 16S rRNA. Se obtuvo 41 aislados, de los cuales ocho presentaron inhibición del crecimiento In Vitro de P. infestans. Se seleccionó el aislado M46 para las pruebas de antagonismo en placas de agar tomate enmendadas con 200 µL de caldo fermentado y se alcanzó un porcentaje de inhibición del 95,4% del crecimiento de P. infestans. El aislado M46 se identificó como Bacillus sp con un porcentaje de identidad del 85% con B. cereus. La cepa de Bacillus sp M46 tiene potencial como agente de control para el patógeno P. infestans.</dc:description><dc:date>2014</dc:date><dc:type>text (article)</dc:type><dc:format>application/pdf</dc:format><dc:identifier>https://dialnet.unirioja.es/servlet/oaiart?codigo=6117852</dc:identifier><dc:identifier>(Revista) ISSN 1909-9959</dc:identifier><dc:identifier>(Revista) ISSN 1692-3561</dc:identifier><dc:source>Biotecnología en el Sector Agropecuario y Agroindustrial: BSAA, ISSN 1692-3561, Vol. 12, Nº. 1 (Enero a Junio), 2014, pags. 28-35</dc:source><dc:language>spa</dc:language><dc:rights>LICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI</dc:rights></oai_dc:dc> |
| id | dialnet-ART0001218921 |
| institution | Dialnet |
| language | spa |
| last_indexed | 2026-10-16T04:41:10Z |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| record_format | oai_dc |
| spelling | dialnet-ART00012189212026-05-15Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de baryOtero, Ivan DarioHurtado Benavides, Andrés MauricioArango Bedoya, OscarFerandez, PabloMartinez, Fernando JavierParra, Zaira StephanieAntagonismOomyceteBacillus spBauer-Kirby techniqueAntagonismoOomyceteBacillus spTécnica de Bauer-KirbyPhytophthora infestans (Mont.) de Bary, has caused considerable losses in all areas where are cultivated the Solanaceae. Its control has been done by chemical, biological, and traditionally by farmers using sisal juice. We isolated bacteria present in the sisal juice. These were tested for antagonism to P. infestans using the principle of Bauer-Kirby technique. The isolate with most inhibition zone was submeted to fermentation in sterile sisal juice and was also tested for antagonism against P. infestans on tomato agar amended with the fermented broth. Finally, we performed the molecular characterization of isolates selected using the 16S rRNA gene ribosomal. We obtained a total of 41 isolates, eigth of which showed good growth inhibition in vitro of P. infestans. The M46 Isolate was selected for testing of antagonism in tomato agar plates amended with 200μl of fermented broth and reached a rate of 95,4% inhibition of growth of P. infestans. The M46 isolate was identified as Bacillus sp M46 with a percentage identity of 85% with Bacillus cereus. The strain of Bacillus sp. M46 has potential as a control agent for the pathogen P. infestans.Phytophthora infestans (Mont.) de Bary, causa pérdidas considerables en todas las áreas donde se cultivan Solanáceas. Su manejo se realiza por métodos químicos, biológicos y tradicionalmente por los agricultores utilizando jugo de fique. Se aislaron bacterias presentes en el jugo de fique y se evaluó el antagonismo contra P. infestans empleando el principio de la técnica de Bauer–Kirbyel. Se seleccionó el aislado con mayor inhibición para realizar una fermentación en jugo de fique estéril y pruebas de antagonismo contra P. infestans en cajas de agar tomate enmendadas con caldo fermentado. Finalmente, se realizó la caracterización molecular del aislado seleccionado mediante la secuencia parcial del gen ribsomal 16S rRNA. Se obtuvo 41 aislados, de los cuales ocho presentaron inhibición del crecimiento In Vitro de P. infestans. Se seleccionó el aislado M46 para las pruebas de antagonismo en placas de agar tomate enmendadas con 200 µL de caldo fermentado y se alcanzó un porcentaje de inhibición del 95,4% del crecimiento de P. infestans. El aislado M46 se identificó como Bacillus sp con un porcentaje de identidad del 85% con B. cereus. La cepa de Bacillus sp M46 tiene potencial como agente de control para el patógeno P. infestans.2014text (article)application/pdfhttps://dialnet.unirioja.es/servlet/oaiart?codigo=6117852(Revista) ISSN 1909-9959(Revista) ISSN 1692-3561Biotecnología en el Sector Agropecuario y Agroindustrial: BSAA, ISSN 1692-3561, Vol. 12, Nº. 1 (Enero a Junio), 2014, pags. 28-35spaLICENCIA DE USO: Los documentos a texto completo incluidos en Dialnet son de acceso libre y propiedad de sus autores y/o editores. Por tanto, cualquier acto de reproducción, distribución, comunicación pública y/o transformación total o parcial requiere el consentimiento expreso y escrito de aquéllos. Cualquier enlace al texto completo de estos documentos deberá hacerse a través de la URL oficial de éstos en Dialnet. Más información: https://dialnet.unirioja.es/info/derechosOAI | INTELLECTUAL PROPERTY RIGHTS STATEMENT: Full text documents hosted by Dialnet are protected by copyright and/or related rights. This digital object is accessible without charge, but its use is subject to the licensing conditions set by its authors or editors. Unless expressly stated otherwise in the licensing conditions, you are free to linking, browsing, printing and making a copy for your own personal purposes. All other acts of reproduction and communication to the public are subject to the licensing conditions expressed by editors and authors and require consent from them. Any link to this document should be made using its official URL in Dialnet. More info: https://dialnet.unirioja.es/info/derechosOAI |
| spellingShingle | Antagonism Oomycete Bacillus sp Bauer-Kirby technique Antagonismo Oomycete Bacillus sp Técnica de Bauer-Kirby Otero, Ivan Dario Hurtado Benavides, Andrés Mauricio Arango Bedoya, Oscar Ferandez, Pablo Martinez, Fernando Javier Parra, Zaira Stephanie Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary |
| title | Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary |
| title_full | Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary |
| title_fullStr | Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary |
| title_full_unstemmed | Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary |
| title_short | Bacterias aisladas del jugo de fique con actividad antagónica sobre phytophthora infestans (mont.) de bary |
| title_sort | bacterias aisladas del jugo de fique con actividad antagonica sobre phytophthora infestans mont de bary |
| topic | Antagonism Oomycete Bacillus sp Bauer-Kirby technique Antagonismo Oomycete Bacillus sp Técnica de Bauer-Kirby |
| url | https://dialnet.unirioja.es/servlet/oaiart?codigo=6117852 |
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