Caracterización del proceso de metaciclogénesis in vitro de promastigotes de Leishmania (Leishmania) mexicana
Introduction. Leishmaniasis is a spectrum of vector-borne diseases caused by protozoa of the genus Leishmania sp. In Mexico, species of the Leishmania (L.) mexicana complex are endemic and cause cutaneous leishmaniasis. During the development of the parasite in the vector and in culture, the pro...
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| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | español |
| Publicado: |
2023
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| Materias: | |
| Acceso en línea: | https://dialnet.unirioja.es/servlet/oaiart?codigo=8795198 |
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| Sumario: | Introduction. Leishmaniasis is a spectrum of vector-borne diseases
caused by protozoa of the genus Leishmania sp. In Mexico, species
of the Leishmania (L.) mexicana complex are endemic and cause
cutaneous leishmaniasis. During the development of the parasite in
the vector and in culture, the process of metacyclogenesis has been
described, which consists of morphological and biochemical changes
for the transformation of procyclic promastigotes into metacyclic
promastigotes.
Objective. To characterize the process of metacyclogenesis in axenic
cultures of Leishmania (L.) mexicana.
Materials and methods. In promastigote cultures of L. (L.) mexicana,
different parameters were evaluated daily for 15 days to determine
changes over time. Cellularity, morphology, and cell viability
parameters were analyzed by optical microscopy and flow cytometry.
Results. In the growth curve, three stages were identified. a) Logarithmic
phase, from day 0 to 6, procyclic promastigotes predominate, they are
larger, viable, metabolically active and have a high replication rate;
b) stationary phase, from day 7 to 10, metacyclic promastigotes are
abundant, they are thin and elongated, there is a proportion of the
parasites with loss of viability; c) death phase, from day 11, there is a
decrease in cell density and the remaining parasites present apoptotic
characteristics.
Conclusion. The characterization of the growth curve of L. (L.)
mexicana allows to identify easily, quickly, and at low-cost, the time
when there is a higher proportion of metacyclic promastigotes in culture, reducing the variability in the results of in
vitro and in vivo experiments. |
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