Perfil proteico de aislados de Leishmania mexicana con diferente virulencia.
Introduction. L. mexicana can cause two clinical forms of cutaneous leishmaniasis: localized (LCL) and diffuse (DLC). Gene expression in trypanosomatids is highly regulated at the post-transcriptional level, a massive and systematic analysis of proteins is a good approach to understand better th...
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| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | español |
| Publicado: |
2023
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| Materias: | |
| Acceso en línea: | https://dialnet.unirioja.es/servlet/oaiart?codigo=8795199 |
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| Sumario: | Introduction. L. mexicana can cause two clinical forms of cutaneous
leishmaniasis: localized (LCL) and diffuse (DLC). Gene expression in
trypanosomatids is highly regulated at the post-transcriptional level,
a massive and systematic analysis of proteins is a good approach to
understand better those mechanisms that parasites have developed for
increase survival and to become more pathogenic in the human host.
Objective. To analyze the protein profile of L. mexicana isolates that
produce LCL and DCL.
Material and methods. Total protein extracts from 10 isolates
of promastigotes in stationary phase were used and processed in
a Q-Exactive plus mass spectrometer, bioinformatic analysis was
performed.
Results. 779 proteins were identified, of which 57 were differentially
expressed and were used for enrichment analysis, representing the
pathways of aminoacylase activity, hydrolase activity, those associated
with binding to cofactors, and chaperonins.
Conclusion. The proteins, FKBP and HSP60, can be studied for their
clinical potential are due to the importance for parasite survival. |
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