T-Cell Receptor: From T-Cell Function to T-Cell Clonality
<p id="p1">Evaluation of T cell clonality has been costly and/or time-consuming. The analysis of TCR β-chain constant region 1 (TRBC1) provides a simplified immunophenotypic assessment of T-cell clonality. Furthermore, due to the high variability of T-cell populations, there is a need for relia...
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| Autores principales: | , |
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| Formato: | Capítulo de libro |
| Lenguaje: | inglés |
| Publicado: |
IntechOpen
2024
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| Materias: | |
| Acceso en línea: | https://www.intechopen.com/chapters/1179656 |
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| Sumario: | <p id="p1">Evaluation of T cell clonality has been costly and/or time-consuming. The analysis of TCR β-chain constant region 1 (TRBC1) provides a simplified immunophenotypic assessment of T-cell clonality. Furthermore, due to the high variability of T-cell populations, there is a need for reliable and robust panels to sort normally from pathological T-cells. The CD27 and CD45RA phenotypic profiling strategy associated with the evaluation of TCRCBeta1 in the same cytometry tube is able to separate normal T Cell populations from clonal populations, gating clusters of cells according to their CD45RA x CD27 expression and then evaluate their TCRCBeta1 status. TCRCBeta1 marker is not only easily implemented in routine immunophenotyping but is also faster and much cheaper than the analysis of TCR-VBeta families either by PCR or by flow cytometry.</p> |
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| ISBN: | 978-0-85466-863-2 |
| DOI: | 10.5772/intechopen.1004631 |