Square-Wave Electric Impulses of 10 ms and 100 V/cm of Field Force, Produced by PGen-1 Impulse Generator Device, Affect the Proliferation Patterns of Different Animal Cells
The influence of the medium-strength electric forces (MSE) on the proliferation of adherent chicken embryo fibroblasts (CEF), VERO, MDBK, MRC-5, and HeLa; lymphoblast cells, FB1 and K562; and cell multiplications were analyzed by growth index (GI). Impulse generator device PGen-1 provided 100 V/cm s...
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| Main Authors: | , , , , , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
IntechOpen
2020
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| Subjects: | |
| Online Access: | https://www.intechopen.com/chapters/70555 |
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| Summary: | The influence of the medium-strength electric forces (MSE) on the proliferation of adherent chicken embryo fibroblasts (CEF), VERO, MDBK, MRC-5, and HeLa; lymphoblast cells, FB1 and K562; and cell multiplications were analyzed by growth index (GI). Impulse generator device PGen-1 provided 100 V/cm square-wave impulses of 10 ms. Treatment: Samples were subjected to one or three MSE. GIs were compared with controls after 72 hours and one or three treatments: Monolayers: CEF: GI in the control is 16.76, and after one and three MSE, it is 15.81 and 7.09. Vero cells: GI in the controls is 8.39, and after one and three MSE, it is 5.39 and 5.69. MDBK cells: GI in controls is 8.39, and after one and three MSE, it is 5.39 and 5.69. MRC-5 cells: GI in controls is 5.58, and after one and three MSE, it is 4.18 and 2.60. HeLa cells: GI in controls is 13.69, and after one and three MSE, it is 10.16 and 5.37. Suspension cells: Lymphoblast FB1: GI in controls is 6.55, and after one and three MSE, it is 13.48 and 12.25. Lymphoblast K562: GI in controls is 9.07, and after one or three MSE, it is 12.37 and 13.55. To conclude: MSE in monolayer cells inhibits the GI, depending on the nature of cells. MSE enhances the multiplication of lymphoblast FB1 or K562. |
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| ISBN: | 978-1-78984-518-1 |
| DOI: | 10.5772/intechopen.90506 |