Library Preparation for Whole Genome Bisulfite Sequencing of Plant Genomes
<p id="p1">Epigenetic mechanisms are a key interface between the environment and the genotype. These mechanisms regulate gene expression in response to plant development and environmental stimuli, which ultimately affects the plant’s phenotype. DNA methylation, in particular cytosine methylati...
Guardado en:
| Autores principales: | , |
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| Formato: | Capítulo de libro |
| Lenguaje: | inglés |
| Publicado: |
IntechOpen
2020
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| Materias: | |
| Acceso en línea: | https://www.intechopen.com/chapters/70771 |
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| Sumario: | <p id="p1">Epigenetic mechanisms are a key interface between the environment and the genotype. These mechanisms regulate gene expression in response to plant development and environmental stimuli, which ultimately affects the plant’s phenotype. DNA methylation, in particular cytosine methylation, is probably the best studied epigenetic modification in eukaryotes. It has been associated to the regulation of gene expression in response to cell/tissue differentiation, organism development and adaptation to changing environments. Whole genome bisulfite sequencing (WGBS) is considered the gold standard to study DNA methylation at a genome level. Here we present a protocol for the preparation of whole genome bisulfite sequencing libraries from plant samples (grapevine leaves) which includes detailed instructions for sample collection and DNA extraction, sequencing library preparation and bisulfite treatment.</p> |
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| ISBN: | 978-1-83880-817-4 |
| DOI: | 10.5772/intechopen.90716 |