Special Cuvettes for Spectrofluorimeters
Special cuvettes that dramatically increase the fluorescence intensity of diluted solutions and suspensions of isolated biomembranes and living cells are described. (1) Mirror cuvettes have a reflecting aluminum or silver layer, putting on their three external sides, having a slot-hole window at fro...
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| Autor principal: | |
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| Formato: | Capítulo de libro |
| Lenguaje: | inglés |
| Publicado: |
IntechOpen
2020
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| Acceso en línea: | https://www.intechopen.com/chapters/72344 |
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| Sumario: | Special cuvettes that dramatically increase the fluorescence intensity of diluted solutions and suspensions of isolated biomembranes and living cells are described. (1) Mirror cuvettes have a reflecting aluminum or silver layer, putting on their three external sides, having a slot-hole window at frontal side center. (2) Mirror microcuvettes have two reflecting sides. (3) Total internal reflection cuvettes have lateral reflecting side and two triangular prisms, attached outside; the cuvette works effectively with a parallel excitation light beam. (4) In cuvette with diagonal quartz plate, very small quantity of a sample is attached in the form of a thin layer. (5) Mirror cuvette with diagonal plate. All described cuvettes can be applied for measurements of spectra excitation and emission, excited-state lifetime, and polarization degree. Also, they can be used for photo-bleaching of biological chromophores. |
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| ISBN: | 978-1-83968-039-7 |
| DOI: | 10.5772/intechopen.92720 |