A Protocol for Minimal Single Protein Labeling with CyDye Fluors for Live Cell Internalization Assays

Individual proteins chemically labeled with fluorescent dyes can be localized and tracked in real-time experiments in order to get insights about the site and molecular mechanism of action. Here, we have adapted a protocol that was originally developed for two-dimensional fluorescence difference gel...

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Autores principales: Castro, Antonio J., Clemente, Alfonso, Alché, Juan de Dios
Formato: Capítulo de libro
Lenguaje:inglés
Publicado: IntechOpen 2020
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Acceso en línea:https://www.intechopen.com/chapters/72503
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