Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach
Escherichia coli is a Gram-negative bacteria which is well known for its pathogenic properties that can cause serious food poisoning, mostly indicated by diarrhea or other severe symptoms. Despite of its well-known properties due to its ability to produce toxin, most of E. coli strains are harmless...
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| Formato: | Capítulo de libro |
| Lenguaje: | inglés |
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IntechOpen
2020
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| Acceso en línea: | https://www.intechopen.com/chapters/72002 |
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| _version_ | 1879164702422990848 |
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| author | Maksum, Iman Permana Nabiel, Ahmad Ishmayana, Safri Soedjanaatmadja, Ukun M.S. |
| author_browse | Ishmayana, Safri Maksum, Iman Permana Nabiel, Ahmad Soedjanaatmadja, Ukun M.S. |
| author_facet | Maksum, Iman Permana Nabiel, Ahmad Ishmayana, Safri Soedjanaatmadja, Ukun M.S. |
| author_sort | Maksum, Iman Permana |
| building | IntechOpen |
| collection | Acceso abierto |
| description | Escherichia coli is a Gram-negative bacteria which is well known for its pathogenic properties that can cause serious food poisoning, mostly indicated by diarrhea or other severe symptoms. Despite of its well-known properties due to its ability to produce toxin, most of E. coli strains are harmless and even beneficial especially in recombinant protein production. This bacterium is suitable for protein recombinant host since it has rapid growth, high expression rate, and well-known genome. Various proteins have been produced using E. coli expression systems, with therapeutic protein for medical application being the most notably produced. Apart from that, our group succeeded in producing beta galactosidase from a wild type E. coli strain B130. Furthermore, recombinant human serum albumin was successfully produced using E. coli strains BL21 (DE3). However, studies on E. coli toxin contamination in recombinant protein productions, strains, and genomic comprehension are indispensable, particularly in therapeutic protein. Therefore, this chapter will discuss the safety aspects of recombinant therapeutic proteins in terms of toxin contamination by strain and genomic approaches. |
| doi_str_mv | 10.5772/intechopen.92031 |
| first_indexed | 2026-10-15T21:38:32Z |
| format | Book Chapter |
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<dc:title>Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach</dc:title>
<dc:creator>Iman Permana Maksum</dc:creator>
<dc:creator>Ahmad Nabiel</dc:creator>
<dc:creator>Safri Ishmayana</dc:creator>
<dc:creator>Ukun M.S. Soedjanaatmadja</dc:creator>
<dc:subject>Health Sciences</dc:subject>
<dc:description>Escherichia coli is a Gram-negative bacteria which is well known for its pathogenic properties that can cause serious food poisoning, mostly indicated by diarrhea or other severe symptoms. Despite of its well-known properties due to its ability to produce toxin, most of E. coli strains are harmless and even beneficial especially in recombinant protein production. This bacterium is suitable for protein recombinant host since it has rapid growth, high expression rate, and well-known genome. Various proteins have been produced using E. coli expression systems, with therapeutic protein for medical application being the most notably produced. Apart from that, our group succeeded in producing beta galactosidase from a wild type E. coli strain B130. Furthermore, recombinant human serum albumin was successfully produced using E. coli strains BL21 (DE3). However, studies on E. coli toxin contamination in recombinant protein productions, strains, and genomic comprehension are indispensable, particularly in therapeutic protein. Therefore, this chapter will discuss the safety aspects of recombinant therapeutic proteins in terms of toxin contamination by strain and genomic approaches.</dc:description>
<dc:publisher>IntechOpen</dc:publisher>
<dc:date>2020-05-01</dc:date>
<dc:type>Chapter, Part Of Book</dc:type>
<dc:identifier>https://www.intechopen.com/chapters/72002</dc:identifier>
<dc:identifier>doi:10.5772/intechopen.92031</dc:identifier>
<dc:language>en</dc:language>
<dc:relation>ISBN:978-1-83962-523-7</dc:relation>
<dc:rights>https://creativecommons.org/licenses/by/3.0/</dc:rights>
<dc:source>https://www.intechopen.com/books/8722 ; E. Coli Infections - Importance of Early Diagnosis and Efficient Treatment</dc:source>
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| id | intech-72002 |
| institution | IntechOpen |
| isbn | 978-1-83962-523-7 |
| language | English |
| last_indexed | 2026-10-15T21:38:32Z |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | IntechOpen |
| publisherStr | IntechOpen |
| record_format | oai_dc |
| spelling | intech-720022020-05-01 Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach Iman Permana Maksum Ahmad Nabiel Safri Ishmayana Ukun M.S. Soedjanaatmadja Health Sciences Escherichia coli is a Gram-negative bacteria which is well known for its pathogenic properties that can cause serious food poisoning, mostly indicated by diarrhea or other severe symptoms. Despite of its well-known properties due to its ability to produce toxin, most of E. coli strains are harmless and even beneficial especially in recombinant protein production. This bacterium is suitable for protein recombinant host since it has rapid growth, high expression rate, and well-known genome. Various proteins have been produced using E. coli expression systems, with therapeutic protein for medical application being the most notably produced. Apart from that, our group succeeded in producing beta galactosidase from a wild type E. coli strain B130. Furthermore, recombinant human serum albumin was successfully produced using E. coli strains BL21 (DE3). However, studies on E. coli toxin contamination in recombinant protein productions, strains, and genomic comprehension are indispensable, particularly in therapeutic protein. Therefore, this chapter will discuss the safety aspects of recombinant therapeutic proteins in terms of toxin contamination by strain and genomic approaches. IntechOpen 2020-05-01 Chapter, Part Of Book https://www.intechopen.com/chapters/72002 doi:10.5772/intechopen.92031 en ISBN:978-1-83962-523-7 https://creativecommons.org/licenses/by/3.0/ https://www.intechopen.com/books/8722 ; E. Coli Infections - Importance of Early Diagnosis and Efficient Treatment |
| spellingShingle | Health Sciences Maksum, Iman Permana Nabiel, Ahmad Ishmayana, Safri Soedjanaatmadja, Ukun M.S. Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach |
| title | Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach |
| title_full | Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach |
| title_fullStr | Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach |
| title_full_unstemmed | Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach |
| title_short | Safety Aspect of Recombinant Protein Produced by <em>Escherichia coli</em>: Toxin Evaluation with Strain and Genomic Approach |
| title_sort | safety aspect of recombinant protein produced by em escherichia coli em toxin evaluation with strain and genomic approach |
| topic | Health Sciences |
| url | https://www.intechopen.com/chapters/72002 |
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