Molecular and Functional Analysis of Soybean Allergen Proteins with a Focus on Pollen-Food Syndrome
<p id="p2">Gly m 3 and Gly m 4 are major soybean food allergens, causing birch pollen cross-allergic reactions, particularly with Bet v 1 and Bet v 2. These allergens can mediate anaphylactic reactions; however, the causative factors are still unknown. The goals of this comparative study are to char...
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| Autores principales: | , , , , , , |
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| Formato: | Capítulo de libro |
| Lenguaje: | inglés |
| Publicado: |
IntechOpen
2024
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| Materias: | |
| Acceso en línea: | https://www.intechopen.com/chapters/89623 |
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| Sumario: | <p id="p2">Gly m 3 and Gly m 4 are major soybean food allergens, causing birch pollen cross-allergic reactions, particularly with Bet v 1 and Bet v 2. These allergens can mediate anaphylactic reactions; however, the causative factors are still unknown. The goals of this comparative study are to characterize (A) the structural functionality of Gly m allergens of <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">Glycine max</italic></em> and Bet v allergens of <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">Betula pendula</italic></em> form birch, with a focus on their immunological properties, and (B) the molecular mechanisms of cross-allergenicity involved in pollen-food syndrome. This was achieved by extensive analysis using different molecular computer-aided approaches covering (1) physicochemical properties and functional-regulatory motifs, (2) sequence analysis, 2D and 3D structural homology modeling comparative study, (3) conservational and evolutionary analysis, (4) identification of B-cell epitopes based on sequence and structure-docking, while T-cell epitopes were identified by inhibitory concentration and binding score methods. Thus, we found that particular epitopes, in addition to the conserved ones, could be responsible for eliciting cross-reactivity between Bet v 1 and Bet v 2, and their respective homolog allergens proteins found in soybean. Moreover, variable epitopes were present in the Gly m 4 and Gly m 3 structures, which may be also responsible for this causative cross-allergenicity between soybean seed and birch pollen proteins.</p> |
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| ISBN: | 978-0-85014-222-8 |
| DOI: | 10.5772/intechopen.115283 |