Can Lactobacilli be Used as Probiotics? – An Insight
<p id="p1">Lactic acid bacteria (LAB) are widely used in the food and health industries due to their Generally Recognized as Safe (GRAS) status and Qualified Presumption of Safety (QPS) designation. However, recent research has revealed that some <em><italic xmlns:mml="http://www.w3.org/1998/Math/Ma...
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| Main Authors: | , |
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| Format: | Book Chapter |
| Language: | English |
| Published: |
IntechOpen
2025
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| Subjects: | |
| Online Access: | https://www.intechopen.com/chapters/89791 |
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| Summary: | <p id="p1">Lactic acid bacteria (LAB) are widely used in the food and health industries due to their Generally Recognized as Safe (GRAS) status and Qualified Presumption of Safety (QPS) designation. However, recent research has revealed that some <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">Lactobacillus</italic></em> species harbor antimicrobial-resistance genes, which may contribute to the rise of antimicrobial resistance (AMR). Genome analysis of LAB, available through resources like the National Centre for Biotechnology Information (NCBI) database, which lists over 13,711 genomes within the Lactobacillaceae family, provides valuable insights into strain selection and gene identification. This genomic data is instrumental in identifying LAB strains that not only offer health benefits, such as immunomodulation, pathogen antagonism, vitamin production (especially B vitamins), and potential anticancer properties, but also carry genes linked to antimicrobial resistance, including penicillin-binding proteins (PBPs). Such insights enable the categorization and safety screening of LAB strains, allowing for safer applications in both the healthcare and food sectors, while also guiding future research on LAB’s role in combatting conditions like obesity and hyperammonemia. In this study, <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">Lactobacillus crispatus</italic></em> strain B4 (CP059140.1), <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">Lactobacillus iners</italic></em> (CP049223.1), and <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">Lactobacillus johnsonii</italic></em> (CP062068.1) were used as representative strains that harbor <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">erm(B)</italic></em> and <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">tet</italic></em> genes. 23S rRNA (adenine(2058)-N(6))-methyltransferase <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">Erm(B)</italic></em> protein and tetracycline-resistant protein of <em><italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">L.crispatus</italic></em> were also predicted, and Ramachandran plot revealed that the structure has 96.86% and 84.43% in the favored regions, respectively.</p> |
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| ISBN: | 978-0-85014-354-6 |
| DOI: | 10.5772/intechopen.115610 |