Evaluación de la producción de ácido láctico a partir de un clúster de microorganismos nativos de una biorrefinería colombiana
In this study, a total of sixteen strains of native lactic acid bacteria were isolated from a Colombian biorefinery, originating from alcoholic fermentation musts (10) and sugar cane molasses (6). This cluster was evaluated for lactic acid production in Man, Rogosa, and Sharpe (MRS) medium (100 mL)...
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| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | español |
| Publicado: |
2024
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| Acceso en línea: | https://dialnet.unirioja.es/servlet/oaiart?codigo=9886724 |
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| Sumario: | In this study, a total of sixteen strains of native lactic acid bacteria were isolated from a Colombian biorefinery, originating from alcoholic fermentation musts (10) and sugar cane molasses (6). This cluster was evaluated for lactic acid production in Man, Rogosa, and Sharpe (MRS) medium (100 mL) under anaerobic conditions, at 150 rpm, and without pH control. The effects of temperature (37, 39.5, 42 °C) and fermentation time (0, 8, and 24 hours) on lactic acid production were assessed. The lactic acid bacteria (LAB) strains that achieved metabolite production comparable to the reference strain Lactobacillus delbrueckii ATCC 9649 were Pediococcus acidilactici and Pediococcus pentosaceus 1, producing 12 gL-1 of lactic acid with kinetic parameters of 0.61 Yp/s and 0.51 gL-1h-1 at 42 °C in 24 hours. Subsequently, these two strains were scaled up to a bioreactor level in a sucrose rich medium at 42 °C, 150 rpm, with 3N NaOH as a pH control agent at 6.8. Lactic acid (LA) production and other metabolites were analyzed during 44 hours of fermentation. Pediococcus acidilactici exhibited the best fermentative performance with a production of 30 gL-1 of lactic acid, 0.66 Yp/s, and 0.68 gL-1h-1. Additionally, yields of 0.13 and 0.04 gg-1 of acetic acid and ethanol, respectively, were produced under the evaluated conditions. |
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